Mahboubeh Tajaldini, Firooz Samadi, Ayyoob Khosravi, Azim Ghasemnejad, Jahanbakhsh Asadi,
Volume 14, Issue 2 (3-2020)
Abstract
ABSTRACT
Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of orange peel extract (OPE) and naringin (NR) on esophageal squamous cell carcinoma (ESCC) cells.
Methods: Amount of phenol, flavonoid and antioxidants in OPE was determined using Folin-Ciocalteu procedure, aluminum chloride colorimetric and DPPH assays, respectively. Effects of NR and OPE on viability, wound healing assay and DNA fragmentation using DAPI were investigated. Data were analyzed by ImageJ software and GraphPad Prism 6.0 at significance of 0.05.
Results: Total amount of phenols, flavonoids and 1,1-diphenyl-2-picrylhydrazyl was 2.83, 2.143 and 60.76 g/100g of OPE. Amount of NR in the dried OPE was estimated to be 5.260 (µg/gr) using high-performance liquid chromatography. Treatment of ESCC cells with OPE or NR decreased viability y of cancer cells in a dose-dependent manner. In addition, both OPE and NR were able to decrease cell migration and increase DNA fragmentation.
Conclusion: The findings of our study suggest that OPE and NR have anticancer effects on ESCC cells but the anticancer effects of OPE was better than that of NR alone.
Keywords: Orange peel extract, Naringin, Migration, Esophageal squamous cell carcinoma.
Seyed Zanyar Athari, Mir Alireza Nourazar, Mohammadreza Nasirzadeh,
Volume 16, Issue 3 (5-2022)
Abstract
Background and objectives: Increased oxidative stress and altered antioxidant levels play an essential role in the pathogenesis of diabetes mellitus. Menopause is associated with increased food intake, weight gain, metabolic disorders, and increased level of inflammatory and oxidative stress factors. This study evaluated effects of oleuropein extract on brain tissue and serum oxidative status in ovariectomized diabetic rats.
Methods: In this study, 40 female Wistar rats weighing 250±20 grams were randomly divided into four groups: control, sham (surgery without ovariectomy), ovariectomy+diabetes, and ovariectomy+diabetes+oleuropein supplementation. Diabetes was induced by intraperitoneal injection of streptozotocin. The rats in the treatment group received 60 mg/kg of oleuropein for 30 days by oral gavage. Finally, blood glucose, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and malonaldehyde levels were evaluated in serum and brain tissue samples.
Results: Weight, blood glucose level, and antioxidant enzymes activity increased significantly in the ovariectomy+diabetes group compared to the control group.
Conclusion: This study suggested that the administration of oleuropein extract has beneficial effects on blood glucose level and antioxidant status in serum and brain tissue of ovariectomized diabetic rats.
Mr Joseph Raymond Enoghase, Dr Silvanus Olu Innih,
Volume 20, Issue 3 (7-2026)
Abstract
Background: Mercury chloride is a well-established toxicant known to induce hematological alterations, including oxidative hemolysis, anemia, and thrombocytopenia. Medicinal plants with antioxidant properties have been explored as potential protective agents against toxicant-induced damage. Anthocleista djalonensis, widely used in traditional medicine, has been reported to possess bioactive compounds with therapeutic potential. This study aimed to investigate the role of Anthocleista djalonensis in mitigating mercury chloride-induced hematological alterations.
Methods: This experimental randomised controlled animal study was kind of weirdly done, with thirty-six adult Wistar rats, randomly assigned into six groups, (n=6 each). Group A was basically the control, Group B got only 2 mg/kg of mercury chloride , just that. Groups C and D were given mercury chloride alongside 150 mg/kg and 300 mg/kg of Anthocleista djalonensis extract, respectively , while Groups E and F received just A. djalonensis at doses 150 mg/kg and 300 mg/kg. The extract was freeze dried, and then the haematological parameters were checked using an automated haematology analyser. For the statistics, it was handled with one-way ANOVA, and the significance cut off was p < 0.05.
Results: Mercury chloride exposure significantly reduced red blood cell count, hemoglobin concentration, and platelet levels, indicating hematotoxicity. Co-administration with A. djalonensis, particularly at 150 mg/kg, significantly improved these hematological indices, suggesting a protective effect. The plant extracts also enhanced mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, indicating potential erythropoietic support.
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